NYU Dataset

Staphylococcus Aureus Cell Envelope Selectively Controls the Sorting of Selective Exoproteins

Part of: Torres Lab |
UID: 10568
Description

Leukocidins are crucial components of Staphylococcus aureus (S. aureus) and they are secreted toxins that directly target and lyse immune cells. One of the leukocidins, Leukocidin AB (LukAB), is associated with the bacterial cell envelope. This study examined how S. aureus regulates LukAB secretion as well as the role of bacteria-associated LukAB in host-pathogen interaction. Using tissue culture models of infection, they investigated whether the localization of LukAB on the bacterial cells contributes to S. aureus-mediated killing of primary human neutrophils. The dataset contains immunofluorescence imaging and mass spectrometry data. In addition, the publication includes source data for underlying figures.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
The mass spectrometric raw data generated in this study are accessible from the MassIVE database. The exoprotein mass spectrometry data were obtained from the MassIVE database and the nucleotide sequences of genes were acquired from GenBank through NCBI Nucleotide database. Source data are provided with this paper on PubMed Central (PMC).
Access via MassIVE

Mass spectrometric raw data
Accession #: MSV000086238

Access via MassIVE

Exoprotein mass spectrometry data
Accession #: MSV000080260

Access via GenBank

Nucleotide sequences of genes
Accession #: NC_007793.1

Access via PMC

Source data
Accession #: PMC8548510

Associated Publications
Data Type
Equipment Used
Bio-Rad ChemiDoc
EnVision Multilabel Plate Reader
LI-COR Odyssey CLx
N-SIM S Super Resolution Microscope
RMC MT-X Ultramicrotome
SpeedVac
Thermo Scientific EASY-nLC 1000
Zeiss Leo 906 E
Zeiss Leo 912 AB OMEGA
Zeiss LSM 880
Software Used
Clustal Omega
GraphPad Prism v8.0
HMMER
Image Studio
ImageJ
ImageSP
MATLAB
MaxQuant v1.5.2.8
Microsoft Excel
NIS-Elements
PSORTb v3.0.2
ScanProsite
SignalP v5.0
ZEN
Grant Support
Max Planck Society for the Advancement of Science/Max Planck Society for the Advancement of Science
Burroughs Wellcome Fund/Burroughs Wellcome Fund
Cystic Fibrosis Postdoctoral Research Fellowship/Cystic Fibrosis Foundation