Staphylococcus Aureus Cell Envelope Selectively Controls the Sorting of Selective Exoproteins
- Description
Leukocidins are crucial components of Staphylococcus aureus (S. aureus) and they are secreted toxins that directly target and lyse immune cells. One of the leukocidins, Leukocidin AB (LukAB), is associated with the bacterial cell envelope. This study examined how S. aureus regulates LukAB secretion as well as the role of bacteria-associated LukAB in host-pathogen interaction. They used tissue culture models of infection and found that LukAB is responsible for S. aureus-mediated killing of primary human phagocytes. This shows that retention of LukAB on the bacterial cells provides S. aureus with a pre-synthesized active toxin that kills immune cells. The dataset contains immunofluorescence microscopy, electron microscopy, and mass spectrometry data. This study reveals a multistep secretion system that controls exoprotein storage and protein translocation across the S. aureus cell wall.
Access
- Restrictions
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Free to All
- Instructions
- The mass spectrometric raw data generated in this study are accessible in the MassIVE database. The exoprotein mass spectrometry data were obtained from the MassIVE database. The nucleotide sequences of genes used this manuscript were acquired from NCBI GenBank under Nucleotide.
- Grant Support
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Max Planck Society for the Advancement of Science/Max Planck Society for the Advancement of ScienceBurroughs Wellcome Fund/Burroughs Wellcome FundCystic Fibrosis Postdoctoral Research Fellowship/Cystic Fibrosis Foundation