NYU Dataset

Structure Prediction Modeling Characterized Guided Entry of Tail-Anchored Proteins 3 in Mycobacterium Tuberculosis

Part of: Heran Darwin Lab |
UID: 10493
* Corresponding Author
Description

This study investigated the pathways important for Mycobacterium tuberculosis, bacteria that cause tuberculosis, pathogenesis. They searched the Mycobacterium tuberculosis (M. tuberculosis) genome for open reading frames and identified an operon, Rv3679-Rv3680. This operon is predicted to encode proteins with ATPase activity. Using structure prediction modeling, they found that Rv3679 and Rv3680 have some structural similarity to the guided entry of tail-anchored proteins 3 (Get3). Get3 delivers proteins into the membranes of the endoplasmic reticulum. It is also essential for the normal growth and physiology of some eukaryotes. This study characterized the structures of Rv3679 and Rv3680 and examined if Rv3679 and Rv3680 have a role in M. tuberculosis pathogenesis. The dataset contains coordinate and structure factor of ADP, ATPγS, and AMP-PNP. In addition, the publication includes supplementary data, which contains quantitative mass spectrometry data comparing proteins in total lysates of wild type, bagAB, and complemented M. tuberculosis strains.

Subject of Study
Subject Domain
Keywords

Access

Restrictions
Free to All
Instructions
The coordinate and structure factor files of ADP, ATPγS, and AMP-PNP were deposited in Protein Data Bank (PDB). All other relevant data are available on PubMed Central (PMC) under Supplementary Materials.
Access via PDB

ADP file
Accession #: 6BS3

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ATPγS file
Accession #: 6BS4

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AMP-PNP file
Accession #: 6BS5

Access via PMC

Other data
Accession #: PMC6597400

Associated Publications
Data Type
Equipment Used
BioSpec Mini Bead Beater
MicroCal PEAQ-ITC System
SpeedVac
Thermo Scientific EASY-nLC 1000
Software Used
Andromeda
Coot
iMosflm
MaxQuant
MicroCal PEAQ-ITC Analysis
MolProbity
Phenix
Scala
Grant Support